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The cDNA products were used directly for subsequent experiments or stored in a 80C refrigerator
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Annals of Saudi Medicine , 20 , 377381
The rate-limiting step involves glutamate-cysteine ligase catalyzing the formation of -glutamylcysteine from L-glutamate and L-cysteine, followed by glutathione synthetase adding glycine to form the complete tripeptide
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