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Materials needed Your AHK-Cu vial (lyophilized) Alcohol swabs Bacteriostatic sterile water 3 mL syringes (Luer Lock tip) 25G or 27G needles (Luer Lock)
The 96-well assay plate media was then replaced with 180 l per well of freshly prepared assay media, and the plate was incubated at 37 C, no CO 2 , for 1 h to eliminate residues of carbonic acid
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