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The gel was stained with Coomassie Brilliant Blue G-250 (3 g l 1 in 45% H 2 O, 45% methanol, 10% glacial acetic acid) for 10 min, then destained overnight in destain solution (85% H 2 O, 10% glacial acetic acid, 5% ethanol)

Tuelings, H.-E

Finally, we performed transmission electron microscopy (TEM) to detect autophagic vesicles at the ultrastructural level

To mitigate false positive detections resulting from multiple t -tests, a control mechanism was introduced to maintain the average false positive detection rate below 0.05, as defined by a specific formula

This naturally occurring antioxidantglutamine, cysteine, and glycineis essential for protecting cells against oxidative damage