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For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)
Residual solvent analysis via gas chromatography ensures that manufacturing solvents are reduced to safe levels well below pharmacopeial limits
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A shared decision-making conversation should address expected glucose reduction targets, monitoring schedules, symptom management strategies, and clear safety thresholds for stopping one or both agents if adverse effects emerge
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