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The quantity and purity of total RNA were then controlled by NanoDrop ND-1000, RNA integrity was tested by Bioanalyzer 2100, and RNA concentration was quantified by agarose electrophoresis

Thereafter, batches of 3 islets per condition were transferred into a 96-well plate containing 200 L KRB per well followed by the addition of 2.8 and 16.7 mM glucose (final concentrations) and the plate was incubated at 37 C for 1 h

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