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The investigators used telomerase-negative human fetal fibroblast lines, treating with Epithalon at multiple concentrations and measuring telomerase activity by TRAP assay and telomere length by Southern blot

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Third, the K m value (Michaelis constant) of laccase for O 2 (2050 M) 16 is in the range of physiological O 2 concentrations in tissue (2964 M in the human brain for example 17 ), suggesting potential in vivo applicability