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The chromatographic column can be a C 18 chromatographic column (Kromasil-C 18 , 30mm*250mm, Hanbang Technology Chromatography Co., Ltd.), and the samples are loaded in multiple times, and the effluents of the target peaks are collected respectively

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This was because hydroxocobalamin produced by bacteria was changed to cyanocobalamin during purification in activated charcoal columns after separation from the bacterial cultures (because cyanide is naturally present in activated charcoal)

Good Candidates Athletes recovering from tendon/ligament injuries Achilles tendonitis, rotator cuff issues, tennis elbow, patellar tendinopathy