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The islets were then fixed with 4% paraformaldehyde in PBS for 30 minutes, permeabilized with 0.25% Triton-X for 7 minutes, and blocked in blocking solution (2% Glycine, 2% BSA, 5% FBS, 50 mM NH 4 Cl in PBS) for 1 hour
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Lancet 399(10322):394405
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