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For full mechanistic comparison see BPC-157 vs TB-500: Tissue Repair Research Comparison

1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

You'll find GHK-Cu as an active ingredient in skin care products like serums and creams for mature or acne-prone skin, as the compound supports elastin and collagen production, which are proteins that are integral to the structure of the skin [1]

coli BL21(DE3) cells, harbouring the recombinant plasmid, were cultivated in 2xYT medium (supplemented with 0.1 mg ml 1 ampicillin) at 37 C until OD 600 reached ~0.8 and then cooled to 18 C for 30 min

This will give you a clearer picture of why it has gained so much attention and what the current evidence actually suggests