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After the reaction was completed, an appropriate amount of a mixed solution of acetic anhydride and pyridine (volume ratio: Ac2O/Pyridine=7/6) was added to block the reaction for 5 h or more, following washing three times with DMF
The subcutaneous tissue in these areas provides appropriate depth for medication depot formation, allowing for the sustained release profile necessary for once-weekly dosing
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The relatively moderate increases after surgery are likely related to the small glucose load
To enhance the cell permeability of RT, we converted the carboxylic acid groups to acetoxymethyl (AM) esters, which are readily hydrolysed by esterases to regenerate RT inside cells
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