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pylori was grown on Columbia blood agar (Difco) plates containing 5% (v/v) defibrinated horse blood (Hemostat Labs), 50 g/mL cycloheximide (Sigma), 10 g/mL vancomycin (Sigma), 5 g/mL cefsulodin (Sigma), 2.5 U/mL polymyxin B (Sigma), 5 g/mL trimethoprim (Sigma), 8 g/mL amphotericin B (Fisher Scientific), 0.2% (w/v) -cyclodextrin (Sigma), and 25 g/mL chloramphenicol (Sigma) or 50 g/mL kanamycin (Fisher Scientific) as needed at 37C in a humidified 10% CO 2 incubator for 23 days
Stacking Semax with a bedtime peptide requires a split schedule, not a modified dose
The common starting dose in community protocols is 100 mcg per shot, once daily at bedtime, on an empty stomach, for about one week
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