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three times daily adds a midday dose
Isolation of RNA and RT-qPCR Total RNA was extracted using the RNeasy mini kit (Qiagen, Valencia, CA), and then quantified with the Nanodrop 1000 (Thermo Fisher Scientific, Inc., Waltham, MA), followed by cDNA synthesis (Qiagen) and RT-qPCR
Breeding range, migration, wintering range, habitat The breeding range of Pectoral Sandpiper extends from the central Canadian Arctic north and west of Hudson Bay to northern and western Alaska and more than 3,000 miles (5,000 km) west along the Siberian Arctic coast
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The IMR is independent of Kaiser and decisions are binding
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