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Separated single neurons were cultured in Eagles MEM with 10% fetal bovine serum supplemented with 5.6 mM glucose for 1224 h
This has been confirmed using HPLC analysis and is documented in a research paper published in GSC Biological and Pharmaceutical Sciences in 2026
10.1111/j.1463-1326.2012.01557.x 64 JiL
The 96-well assay plate media was then replaced with 180 l per well of freshly prepared assay media, and the plate was incubated at 37 C, no CO 2 , for 1 h to eliminate residues of carbonic acid
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