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Aromatase inhibitors, a common class of breast cancer medication, seem to limit the effectiveness of GLP-1 drugs, perhaps due to their anti-estrogen effects
But if there isnt enough ascorbic acid available, the collagen strands are underhydroxylated and instead of forming strong tropocollagen, the underhydroxylated collagen is degraded as shown below
et al (2021) Structural basis of TRAPPIII-mediated Rab1 activation
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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles
