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After incubation in the dark at 4 C for 2 days, plates were cultivated in a growth chamber at 22 C with a 16 h/8 h light/dark (long day, LD) photoperiod

The tumor growth inhibition properties of Ru(II)-OH, NP2, or NP4 (2 mg Ru/kg) were studied upon intravenous injection in the tail vein of 143B tumor bearing mouse models

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The cell pellet was thawed, homogenized, and lysed in a buffer containing 20 mM HEPES pH 7.5, 50 mM NaCl, 2 mM MgCl 2 , and complete protease inhibitor cocktail tablet (Roche), and the membranes were collected by centrifugation at 40,000 g