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The two glucagon-like sequences were rapidly synthesized by several groups, but neither the first, corresponding to the proglucagon 72108 sequence or the second, corresponding to proglucagon 126158 (it was predicted that the two final C-terminal amino acids, both basic, would be cleaved off during processing), had any effect on pancreatic insulin secretion (an early positive report of insulinotropic effects of PG 72108 at very high concentrations (10 7 mol/L) ( Figure 1 A first, the discovery of GLP-1 was met with moderate excitement
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Only proteins identified with at least two unique peptidesexcluding contaminants and reverse hitswere retained for downstream analysis
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