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Before proceeding with the incisions, all animals were anesthetized using an intraperitoneal injection of pentobarbital sodium (5 mg per 100 g of body weight), and their tail skin was sterilized with iodinated polyvinylpyrrolidone
If measuring outcomes like transepidermal water loss, elasticity, or biopsy markers, consistent timing eliminates this confounding variable
Stability: For maximum safety and sterility, discard the vial 28 days after the initial puncture
The reason that night terrors are given such a scary name is that a child experiencing a night terror looks as if shes truly afraid of something
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